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Generación de Parabiotic embriones de pez cebra por fusión quirúrgica de Desarrollar blástulas
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1Division of Hematology/Oncology,Boston Children’s Hospital, 2Harvard Medical School, 3Division of Hematology, Department of Medicine,Brigham and Women’s Hospital, 4Harvard Stem Cell Institute, 5Broad Institute of Massachusetts Institute of Technology, 6Howard Hughes Medical Institute, 7Division of Hematologic Malignancies,Dana-Farber Cancer Institute
Capitoli
- 00:05Titolo
- 01:18Preparation for Surgical Fusion
- 03:10Generation of Parabiotic Zebrafish Embryos by Surgical Fusion of Developing Blastulae
- 05:48Image Acquisition, Processing and Analysis
- 07:50Results: Parabiotic Fusion of Zebrafish Embryos
- 09:28Conclusion
This protocol provides step-by-step instruction on how to generate parabiotic zebrafish embryos of different genetic backgrounds. When combined with the unparalleled imaging capabilities of the zebrafish embryo, this method provides a uniquely powerful means to investigate cell-autonomous versus non-cell-autonomous functions for candidate genes of interest.
