È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 00:38Production of X. laevis Embryo Extract
- 07:13Nuclear Shrinking Assay and Immunofluorescence
- 11:28Results: Nuclear Size Regulation Using Xenopus laevis Embryo Extracts
- 13:12Conclusion
Mechanisms of cellular and intra-cellular scaling remain elusive. The use of Xenopus embryo extracts has become increasingly common to elucidate mechanisms of organelle size regulation. This method describes embryo extract preparation and a novel nuclear scaling assay through which mechanisms of nuclear size regulation can be identified.
