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应用荧光共振能量转移（FRET）审查效应转运效率由<em>贝氏柯克斯体</em> siRNA沉默中
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing
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应用荧光共振能量转移（FRET）审查效应转运效率由<em>贝氏柯克斯体</em> siRNA沉默中

DOI:
10.3791/54210-v

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10:29 min

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July 06, 2016

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, ,
1Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity,University of Melbourne

Capitoli

  • 00:05Titolo
  • 01:02Reverse Transfection of siRNA and Seeding of HeLa 229 Cells
  • 04:25Infection of Reverse Transfected Cells
  • 05:39Addition of BlaM Substrate to Determine Level of Translocation
  • 08:23Results: FRET Measurements of Coxiella burnetti Effector Translocation Efficiency in siRNA Silenced HeLa Cells
  • 09:37Conclusion

Summary

Traduzione automatica

Traduzione automatica

调查细菌病原体和宿主之间的相互作用是生物学研究的一个重要领域。在这里，我们描述了必要的技术测量使用布拉姆基板的siRNA的基因沉默过程中贝氏柯克斯体效应易位。

Tags

FRETEffector TranslocationSiRNA SilencingCoxiella BurnetiiCellular MicrobiologyHost-pathogen InteractionsChlamydiaSalmonellaE. ColiBeta-lactamaseACCM2HeLa CellsTransfection96-well Plate

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