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Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions
DOI:

09:34 min

July 30, 2016

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Capitoli

  • 00:05Titolo
  • 00:43Bacterial Culture and Diet Preparation
  • 02:16Egg-laying Cages
  • 03:13Egg Collection
  • 03:55Egg Dechorionation and Sterile Diet Transfer
  • 05:33Gnotobiotic Fly Generation
  • 06:40Measuring CFU Load and Testing for Sterility
  • 08:05Results: Successful Axenic Fly Rearing Confirmed with CFUs
  • 08:49Conclusion

Summary

Traduzione automatica

A method for rearing Drosophila melanogaster under axenic and gnotobiotic conditions is presented. Fly embryos are dechorionated in sodium hypochlorite, transferred aseptically to sterile diet, and reared in closed containers. Inoculating diet and embryos with bacteria leads to gnotobiotic associations, and bacterial presence is confirmed by plating whole-body Drosophila homogenates.

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