/
/
Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 01:40Enrichment of Robust Derivatives during Serial Transfer on AFEX CSH
- 03:42Isolation of Single Cell Tolerant Derivatives after Enrichment on AFEX CSH
- 04:58Performance Evaluation of AFEX CSH Tolerant Derivatives Compared to Parent
- 07:15Applying Continuous Culture to Select for Ethanol-challenged Xylose Utilization
- 09:43Isolation of Single-cell Colonies That Utilize Xylose in PSGHL When Ethanol Is Present
- 11:20Results: Derivatives of S. stipitis Parent Strain Have Improved Efficiency in Converting Hydrolyzates to Ethanol
- 12:54Conclusion
Adaptive evolution and isolation techniques are described and demonstrated to yield derivatives of Scheffersomyces stipitis strain NRRL Y-7124 that are able to rapidly consume hexose and pentose mixed sugars in enzyme saccharified undetoxified hydrolyzates and to accumulate over 40 g/L ethanol.
