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طريقة سهلة لمحطة Polysome التنميط
JoVE Journal
Biologia
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JoVE Journal Biologia
An Easy Method for Plant Polysome Profiling
DOI:

11:09 min

August 28, 2016

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Capitoli

  • 00:00Titolo
  • 01:34Preparation of Sucrose Gradient
  • 02:51Preparation of Cytosolic Extracts
  • 04:09Polysome Profiling
  • 07:07RNA Extraction
  • 09:17Risultati
  • 10:25Conclusion

Summary

Traduzione automatica

This protocol describes an easy method to extract and fractionate transcripts from plant tissues on the basis of the number of bound ribosomes. It allows a global estimate of translation activity and the determination of the translational status of specific mRNAs.

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