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Preparation of Single-cohort Colonies and Hormone Treatment of Worker Honeybees to Analyze Physiology Associated with Role and/or Endocrine System
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Preparation of Single-cohort Colonies and Hormone Treatment of Worker Honeybees to Analyze Physiology Associated with Role and/or Endocrine System

Preparation of Single-cohort Colonies and Hormone Treatment of Worker Honeybees to Analyze Physiology Associated with Role and/or Endocrine System

DOI:
10.3791/54240-v

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08:53 min

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September 06, 2016

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, ,
1Department of Biological Sciences, Graduate School of Sciences,The University of Tokyo, 2Faculty of Pharmaceutical Sciences,Doshisha Women’s College

Capitoli

  • 00:05Titolo
  • 00:36Preparation of Single-cohort Colonies
  • 02:28Classifying Hypopharyngeal Glands (HPGs)
  • 04:09Injection of Active Ecdysone (20E)
  • 05:24Application of Methoprene, a JH Analogue
  • 06:23Results: mrjp2 and Hbg3 Expression in the HPG of Worker Honeybees
  • 07:48Conclusion

Summary

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Here we describe our detailed protocol for the preparation of single-cohort honeybee colonies – a useful tool for analyzing the role-associated worker physiology. We also describe detailed protocols for treating workers with juvenile hormone and ecdysone to evaluate the involvement of these hormones in the regulation of worker behavior and/or physiology.

Tags

Keywords: Single-cohort ColonyWorker HoneybeesPhysiologyRoleEndocrine SystemWorker BehaviorNewly-emerged WorkersNurse BeesPrecocious ForagersHypopharyngeal Glands (HPGs)

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