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Nephrotoxin Mikroinjektion i zebrafisk til model akut nyreskade
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Biologia
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JoVE Journal Biologia
Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury

Nephrotoxin Mikroinjektion i zebrafisk til model akut nyreskade

DOI:
10.3791/54241-v

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07:58 min

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July 17, 2016

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,
1Center for Zebrafish Research, Department of Biological Sciences,University of Notre Dame, 2Center for Stem Cells and Regenerative Medicine, Department of Biological Sciences,University of Notre Dame

Capitoli

  • 00:05Titolo
  • 00:32Preparation of Tools
  • 02:09Embryo Preparation
  • 03:59Microinjection of Nephrotoxin Solution
  • 06:32Results: Experimental Timeline and Post Injection Edema Assay
  • 07:34Conclusion

Summary

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Traduzione automatica

Nyreskader afholdt fra nefrotoksiner, som omfatter lægemidler, der spænder fra antibiotika til kemoterapeutika, kan resultere i komplekse sygdomme, hvis patogenese forbliver ufuldstændigt forstået. Denne protokol viser, hvordan zebrafisk kan anvendes til sygdom modellering af disse forhold, som kan anvendes til identifikation af renoprotective foranstaltninger.

Tags

ZebrafishAcute Kidney InjuryNephrotoxinGentamicinCisplatinEmbryoMicroinjectionAgaroseE3MatingSpawningEmbryo CollectionIncubationSomite Stage

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