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结合活体荧光显微镜 (IVFM) 和遗传模型研究造血细胞对骨髓生态位的植入动力学
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches
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结合活体荧光显微镜 (IVFM) 和遗传模型研究造血细胞对骨髓生态位的植入动力学

DOI:
10.3791/54253-v

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11:06 min

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March 21, 2017

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, , ,
1Wells Center for Pediatric Research, Department of Pediatrics,Indiana University School of Medicine, 2Indiana Center for Biological Microscopy, Department of Medicine,Indiana University School of Medicine, 3Department of Pediatrics,Indiana University School of Medicine

Capitoli

  • 00:05Titolo
  • 01:26Donor Lysozyme Promoter (Lys)-EGFP Mouse Bone Marrow Cell Preparation for Bone Marrow Cell Transplantation (BMT)
  • 03:16Lys-GFP BMT and Intravital Imaging Preparation
  • 05:23In Vivo High Resolution Mouse Calvarium Imaging
  • 07:15Quantitative Analysis
  • 08:14Results: Representative Kinetics and Localization of Myeloid Cell Engraftment Following BMT
  • 10:10Conclusion

Summary

Traduzione automatica

Traduzione automatica

活体荧光显微镜 (IVFM) 的颅骨是应用与遗传动物模型, 研究造血细胞归巢和植入的骨髓 (BM) 龛。

Tags

Intravital Fluorescent MicroscopyIVFMHematopoietic Cell EngraftmentBone Marrow NichesHematopoietic Cell RegenerationCell-to-cell InteractionsMyeloproliferative DiseaseNotch SignalingEGFP MouseBone Marrow TransplantCell IsolationCell SuspensionTail Vein Injection

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