<em>In Vitro</em> Transcription Assays and Their Application in Drug Discovery

Xiao Yang; Cong Ma

doi:10.3791/54256

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In - vitro - Transkriptionsassays und ihre Anwendung in der Drug Discovery

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Genetica

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JoVE Journal Genetica

In Vitro Transcription Assays and Their Application in Drug Discovery

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In - vitro - Transkriptionsassays und ihre Anwendung in der Drug Discovery

DOI:

10.3791/54256-v

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09:28 min

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September 20, 2016

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Xiao Yang, Cong Ma²

¹School of Environmental and Life Sciences,University of Newcastle, ²Department of Applied Biology and Chemical Technology, The State Key Laboratory of Chirosciences,The Hong Kong Polytechnic University

Capitoli

00:05Titolo
00:51Preparation of RNA Sequencing Gel
02:08Transcription Elongation Pausing Assay
04:30RNA Gel Run and Gel Development
06:46Results: NusA Regulates Transcription Pausing
08:34Conclusion

Summary

Traduzione automatica

In this manuscript, we describe a protocol to functionally examine transcription and the inhibitory activity of antibacterial agents targeting bacterial transcription.

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