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从人类肿瘤异种移植的小鼠细胞的消耗显着改善靶细胞下游分析
JoVE Journal
Medicina
This content is Free Access.
JoVE Journal Medicina
Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells
DOI:

07:10 min

July 29, 2016

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Capitoli

  • 00:05Titolo
  • 00:40Tumor Dissociation
  • 02:06Magnetic Cell Separation Mouse Cell Depletion
  • 04:01Representative Results: Downstream Mouse Cell-free Human Tumor Cell Analyses
  • 05:58Conclusion

Summary

Traduzione automatica

人类肿瘤异种移植是血管,并在体内生长阶段由鼠源细胞浸润。为了规避下游分析过程中造成这些污染的细胞偏差,我们开发了允许通过磁性细胞分选所有小鼠细胞的全面枯竭的方法。

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