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Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope
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Biologia
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JoVE Journal Biologia
Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope

Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope

DOI:
10.3791/54262-v

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12:54 min

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July 17, 2016

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, , , ,
1INSERM U1051, Institut des Neurosciences de Montpellier (INM), Université de Montpellier, 2Université de Nîmes, 3CNRS, IES, UMR 5214, 4Aix-Marseille Université, CNRS, École Centrale Marseille, Institut Fresnel, UMR 7249, 5Montpellier RIO Imaging (MRI)

Capitoli

  • 00:05Titolo
  • 00:57Laser System Startup and Microscope Setup
  • 02:13Temporal Synchronization: Part 1
  • 04:25Temporal Synchronization: Part 2
  • 06:46Spatial Overlap of the Beams
  • 08:42Final Adjustments for CARS Signal Observation
  • 10:32Results: Myelin Sheath and Collagen Fibers Viewed by CARS
  • 12:05Conclusion

Summary

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Coherent anti-Stokes Raman scattering (CARS) microscopy based on inherent vibration of molecule bonds permits label-free chemically selective live cell imaging. This work presents the implementation of a complementary microscopy technique on a standard multiphoton laser scanning microscope based on a femtosecond Ti:sapphire laser and an OPO laser.

Tags

Coherent Anti-Stokes Raman Scattering (CARS)Ti:Sapphire LaserOPO LaserLaser Scanning MicroscopyLabel-free ImagingChemically Selective ImagingLipid-related DiseasesSkin PenetrationSkin DisordersDichroic MirrorNarrow Band Pass FilterPMTFluorescenceSHGDelay LinePhoto DiodeOscilloscope

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