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蓝宝石OPO基于激光的标准激光扫描显微镜：在TI相干反斯托克斯拉曼散射（CARS）系统的实现

JoVE Journal
Biologia

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JoVE Journal Biologia

Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope

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蓝宝石OPO基于激光的标准激光扫描显微镜：在TI相干反斯托克斯拉曼散射（CARS）系统的实现

DOI:

10.3791/54262-v

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12:54 min

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July 17, 2016

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Vasyl Mytskaniuk*¹, Fabrice Bardin*^2,3, Hassan Boukhaddaoui⁵, Herve Rigneault, Nicolas Tricaud

¹INSERM U1051, Institut des Neurosciences de Montpellier (INM), Université de Montpellier, ²Université de Nîmes, ³CNRS, IES, UMR 5214, ⁴Aix-Marseille Université, CNRS, École Centrale Marseille, Institut Fresnel, UMR 7249, ⁵Montpellier RIO Imaging (MRI)

Capitoli

00:05Titolo
00:57Laser System Startup and Microscope Setup
02:13Temporal Synchronization: Part 1
04:25Temporal Synchronization: Part 2
06:46Spatial Overlap of the Beams
08:42Final Adjustments for CARS Signal Observation
10:32Results: Myelin Sheath and Collagen Fibers Viewed by CARS
12:05Conclusion

Summary

Traduzione automatica

根据分子键固有振动相干反斯托克斯拉曼散射（CARS）显微允许无标记化学选择性活细胞成像。这项工作提出了一种基于飞秒钛互补显微技术在标准的多光子激光扫描显微镜实现：蓝宝石激光器和激光OPO。

Tags

Coherent Anti-Stokes Raman Scattering (CARS)Ti:Sapphire Laser OPO Laser Laser Scanning Microscopy Label-free Imaging Chemically Selective Imaging Lipid-related Diseases Skin Penetration Skin Disorders Dichroic Mirror Narrow Band Pass Filter PMT Fluorescence SHG Delay Line Photo Diode Oscilloscope

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