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详细的定性C1498小鼠细胞系及其关联白血病小鼠模型协议

JoVE Journal
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JoVE Journal Ricerca sul cancro

A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model

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详细的定性C1498小鼠细胞系及其关联白血病小鼠模型协议

DOI:

10.3791/54270-v

•

08:00 min

•

October 14, 2016

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Alexia Mopin, Virginie Driss, Carine Brinster²

¹Institut pour la Recherche sur le Cancer de Lille (IRCL),INSERM, UMR-S-1172, Centre de Recherche Jean-Pierre Aubert (JPARC), ²Université de Lille

Capitoli

00:05Titolo
00:53Intravenous C1498 Injection and Retro-orbital Blood Collection
02:15Blood Mononuclear Cells Isolation
03:11Bone Marrow Cells Isolation
04:41Bone Marrow Cells Preparation on Slides and May-Grünwald Giemsa staining
06:06Representative Results: C1498 Acute Myeloid Leukemia Characterization
07:14Conclusion

Summary

Traduzione automatica

该原稿提供了可用于表征在体外和诱导小鼠的注射后急性白血病C1498细胞培养技术的过程。表型和功能分析使用流式细胞仪，免疫荧光显微镜，细胞化学和五月 - 格伦沃尔德姬姆萨染色进行。

Tags

Keywords: C1498 Cells Murine Myelomonocytic Leukemia Acute Leukemia Animal Model Congenic C57 Black 6 Mouse Tail Vein Injection Orbital Sinus Blood Draw Mononuclear Cell Isolation Flow Cytometry Bone Marrow Isolation Leukemia Development Immune Deficiency Chemo And Immunotherapeutic Agents

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