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In Vitro Permeation of FITC-loaded Ferritins Across a Rat Blood-brain Barrier: a Model to Study the Delivery of Nanoformulated Molecules
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In Vitro Permeation of FITC-loaded Ferritins Across a Rat Blood-brain Barrier: a Model to Study the Delivery of Nanoformulated Molecules

In Vitro Permeation of FITC-loaded Ferritins Across a Rat Blood-brain Barrier: a Model to Study the Delivery of Nanoformulated Molecules

DOI:
10.3791/54279-v

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11:13 min

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August 22, 2016

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, , , , ,
1Dipartimento di Scienze Biomediche e Cliniche Luigi Sacco,Università di Milano, 2Dipartimento di Biotecnologie e Bioscienze,Università di Milano-Bicocca

Capitoli

  • 00:05Titolo
  • 01:30Establishing the Blood-brain Barrier Model
  • 05:24Blood-brain Barrier Validation: TEER Measurement and Trans-BBB Flux of FD40
  • 06:20FD40 Flux and Trans-BBB Permeation of FTIC-loaded Ferritins (FnN)
  • 08:21Results: Trans-BBB Flux of Nanoformulated FITC and Confocal Microscopy of RBMECs on Inserts
  • 09:58Conclusion

Summary

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Traduzione automatica

A method to establish an in vitro model of blood-brain barrier based on a co-culture of rat brain microvascular endothelial cells and astrocytes is described and validated. This system proved to be a valid tool to study the effect of nanoformulation on the trans-barrier permeation of fluorescent molecules.

Tags

Keywords: In Vitro PermeationFITC-loaded FerritinsRat Blood-brain BarrierNanoformulated MoleculesDrug DeliveryBBB PermeationFerritin Nano-cagesFITCRat Cortical AstrocytesRat Brain Microvascular Epithelial CellsCell CultureTrypsin EDTACentrifugationCell SeedingCell Confluence

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