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Patch Clamp Recordings on Intact Dorsal Root Ganglia from Adult Rats
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Biochimica
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JoVE Journal Biochimica
Patch Clamp Recordings on Intact Dorsal Root Ganglia from Adult Rats

Patch Clamp Recordings on Intact Dorsal Root Ganglia from Adult Rats

DOI:
10.3791/54287-v

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12:00 min

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September 29, 2016

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, ,
1Department of Oral and Maxillofacial Surgery,University of California, San Francisco, 2Department of Anatomy,University of California, San Francisco

Capitoli

  • 00:05Titolo
  • 00:55Dorsal Root Ganglia Dissection
  • 04:09Dorsal Root Ganglia Digestion
  • 06:11Patch Clamp Recordings
  • 10:05Results: Measurement of Neuronal Excitability and Ligand-induced Currents in Small DRG Neurons
  • 10:59Conclusion

Summary

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This manuscript describes how to prepare intact dorsal root ganglia for patch clamp recordings. This preparation maintains the microenvironment for neurons and satellite glial cells, thus avoiding the phenotypic and functional changes seen using dissociated DRG neurons.

Tags

Patch ClampDorsal Root GangliaAdult RatsPrimary Sensory NeuronsPainIntact PreparationSpinal NerveDorsal RootSatellite Glial CellsIn VivoAnesthesiaVertebral ColumnACSFSpinal CordL4L5EpineuriumRecording ChamberOxygenated ACSFPeristaltic Pump

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