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核酸的分离过滤：一种简单快速的DNA提取方法
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Biologia
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JoVE Journal Biologia
Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method
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核酸的分离过滤：一种简单快速的DNA提取方法

DOI:
10.3791/54289-v

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07:56 min

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August 06, 2016

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, , ,
1Center for Innovation in Global Health Technologies (CIGHT), Department of Biomedical Engineering,Northwestern University, 2Pritzker School of Medicine,The University of Chicago

Capitoli

  • 00:05Titolo
  • 01:08Preparation of FINA Sample Preparation Module
  • 02:27Preparation of qPCR Tube
  • 03:12Preparation of Contrived Specimen
  • 04:00FINA Extraction
  • 05:57Results: FINA Enables Efficient Detection of Proviral HIV DNA from Whole Blood
  • 07:20Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里，我们描述了一个简单，快速的基于纸张的DNA提取的HIV前病毒DNA的全血通过定量PCR检测方法。这种协议可以在检测其他遗传标记或使用替代的扩增方法被扩展为使用。

Tags

Keywords: Filtration IsolationNucleic Acid ExtractionDNA ExtractionRapid DNA ExtractionPaper-based DNA ExtractionFINA MethodPCR AmplificationHIV Proviral DNAQuantitative PCRSample Preparation ModuleBlotting PadParaffin FilmGlass Blood Separator MembraneDouble-coated Polyester Diagnostic TapePCR Tube

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