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高含量<em>体外</em>胰岛β细胞复制发现平台
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Medicina
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JoVE Journal Medicina
A High-content In Vitro Pancreatic Islet β-cell Replication Discovery Platform
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高含量<em>体外</em>胰岛β细胞复制发现平台

DOI:
10.3791/54298-v

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09:35 min

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July 16, 2016

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, , ,
1Department of Medicine, Division of Endocrinology,Stanford University School of Medicine

Capitoli

  • 00:05Titolo
  • 00:57Pancreas Perfusion
  • 01:54Pancreas Dissociation
  • 03:41Islet Purification
  • 05:55Islet Cell Dispersion and Plating and Culture Treatment
  • 08:06Results: Dipyridamole Treatment Induces Beta- But Not Alpha-cell Replication
  • 08:48Conclusion

Summary

Traduzione automatica

Traduzione automatica

对于糖尿病研究领域的关键挑战是要明白，调节胰岛β细胞的复制，并制定刺激β细胞再生方法的分子机制。在此高内涵筛选的方法来识别和评估小分子的β细胞复制促进活性表示。

Tags

In VitroPancreatic Isletβ-cell ReplicationScreening PlatformBeta Cell BiologyRegenerative TherapyDiabetesPancreatic DigestionCollagenase

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