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在微孔聚集尺寸优化人类多能干细胞的基于悬浮心脏分化
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Aggregate Size Optimization in Microwells for Suspension-based Cardiac Differentiation of Human Pluripotent Stem Cells
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在微孔聚集尺寸优化人类多能干细胞的基于悬浮心脏分化

DOI:
10.3791/54308-v

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06:28 min

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September 25, 2016

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, ,
1Institute of Biomaterials and Biomedical Engineering,University of Toronto, 2Department of Comparative Biology and Experimental Medicine,University of Calgary

Capitoli

  • 00:05Titolo
  • 00:54Plating hPSC Aggregates in the Microwell Plate
  • 02:00Cardiac Induction Stage 1
  • 03:06Cardiac Induction Stage 2
  • 04:16Cardiac Induction Stage 3
  • 05:06Results: Characteristics of Differentiated hPSCs
  • 05:44Conclusion

Summary

Traduzione automatica

Traduzione automatica

常规的方法以引发人多能的悬浮聚合基于心脏分化干细胞（hPSCs）的困扰与培养异质相对于聚集体的尺寸和形状。在这里，我们描述了采用微孔产生大小控制HPSC心脏推进的条件下培养聚集心脏分化一个可靠的方法。

Tags

Keywords: Aggregate Size OptimizationMicrowellsSuspension-based Cardiac DifferentiationHuman Pluripotent Stem CellsCentrifugationCell SuspensionMicrowell PlateHypoxic IncubationAggregate HarvestingInduction Medium

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