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三维培养试验探索肿瘤细胞浸润和卫星肿瘤形成
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Medicina
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JoVE Journal Medicina
Three-Dimensional Culture Assay to Explore Cancer Cell Invasiveness and Satellite Tumor Formation
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三维培养试验探索肿瘤细胞浸润和卫星肿瘤形成

DOI:
10.3791/54322-v

•

09:23 min

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August 18, 2016

•

, , ,
1CHU de Québec Research Centre, 2Department of Molecular Medicine,Laval University, 3Department of Surgery,Laval University

Capitoli

  • 00:05Titolo
  • 01:00Pseudo-primary Tumor Collagen Plug Preparation
  • 02:26First Fibrin Gel Layer Preparation and Fibrin Gel Generation
  • 04:03Second Fibrin Gel Layer Preparation and Collagen Plug Sandwiching
  • 05:33Cell Culture Medium Conditions
  • 06:27Results: Representative Tumor Cell 3D Culture, Invasiveness, and Satellite Tumor Formation
  • 07:58Conclusion

Summary

Traduzione automatica

Traduzione automatica

Cancer cells are embedded in a collagen gel and then sandwiched in an acellular fibrin gel to generate a 3D culture system in which the invasiveness and formation of satellite tumors may be monitored.

Tags

Keywords: 3D Cell CultureCollagenFibrin GelCancer Cell InvasivenessSatellite Tumor FormationExtracellular MatrixCell MigrationCell ApoptosisAnti-cancer Drug Evaluation

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