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Continuous-wave Thulium Laser for Heating Cultured Cells to Investigate Cellular Thermal Effects
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JoVE Journal Biologia
Continuous-wave Thulium Laser for Heating Cultured Cells to Investigate Cellular Thermal Effects

Continuous-wave Thulium Laser for Heating Cultured Cells to Investigate Cellular Thermal Effects

DOI:
10.3791/54326-v

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09:49 min

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June 30, 2017

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, , , , , , ,
1Institute of Biomedical Optics,University of Luebeck, 2Medical Laser Center Luebeck GmbH, University of Leubeck, 3Department of Ophthalmology,University of Luebeck

Capitoli

  • 00:05Titolo
  • 01:05Preparation of the Irradiation Station
  • 02:10Temperature Calibration
  • 04:29Thulium Laser Irradiation of a Cell Culture
  • 05:58Assessment of Cell Viability Following Irradiation
  • 07:43Results: Effects of Thermal Irradiation on Cell Viability
  • 08:46Conclusion

Summary

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An original experimental setup for heating cells in a culture dish using 1.94 µm continuous-wave laser radiation is introduced here. Using this method, the biological responses of retinal pigment epithelial (RPE) cells after different thermal exposures can be investigated.

Tags

Keywords: Thulium LaserCell HeatingThermal EffectsIn VitroHyperthermiaRetinal Laser TreatmentMelaninTemperature ControlTemperature Calibration

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