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Bioengineering

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JoVE Journal Bioengineering
Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device
 

Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device

Article DOI: 10.3791/54344-v • 10:51 min • August 30th, 2016
August 30th, 2016 •
1, 1, 1, 2, 2,3,4
1Department of Biomedical Engineering, The University of Texas at Austin, 2Wyss Institute for Biologically Inspired Engineering at Harvard University, 3Vascular Biology Program, Boston Children's Hospital, Harvard Medical School, 4John A. Paulson School of Engineering and Applied Sciences, Harvard University

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We describe an in vitro protocol to co-culture gut microbiome and intestinal villi for an extended period using a human gut-on-a-chip microphysiological system.

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Keywords: Gut-on-a-chip Microfluidic Device Intestinal Villi Microbiome Co-culture Peristalsis-like Motion Extracellular Matrix Ulcerative Colitis Crohn's Disease Host-microbe Interactions Microphysiological System
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