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JoVE Journal
Biologia
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JoVE Journal Biologia
Comparison of Three Different Methods for Determining Cell Proliferation in Breast Cancer Cell Lines
DOI:

12:35 min

September 03, 2016

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Capitoli

  • 00:05Titolo
  • 00:38Preparing Cells for Proliferation Assays
  • 02:49Determining Cell Count Using a Hemocytometer
  • 04:21Determining Cell Proliferation Using Luminescence-based Assay
  • 07:02Determining Cell Count Using a Cell Imager
  • 10:40Results: Comparison of Three Different Methods Measuring Cell Proliferation in Breast Cancer Cell Lines
  • 12:10Conclusion

Summary

Traduzione automatica

This protocol describes the use of three different methods for analyzing cell proliferation in breast cancer cell lines. This includes the use of conventional cell counting, luminescence-based cell viability, and cell counting through the use of a cell imager. Each offers advantages for the reproducible measurement of cell proliferation.

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