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Measuring Lactase Enzymatic Activity in the Teaching Lab
JoVE Journal
Biologia
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JoVE Journal Biologia
Measuring Lactase Enzymatic Activity in the Teaching Lab

Measuring Lactase Enzymatic Activity in the Teaching Lab

DOI:
10.3791/54377-v

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04:41 min

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August 06, 2018

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, , , , ,
1School of Pharmacy,University of Reading, 2Department of Molecular Neuroscience,UCL Institute of Neurology, 3School of Biological Sciences, Royal Hollway,University of London

Capitoli

  • 00:04Titolo
  • 00:23Enzyme Extraction
  • 01:20Observing the Colorimetric Reaction
  • 01:56Measuring Enzyme Activity
  • 03:05Effect of Temperature on Enzyme Activity
  • 03:53Results: Analysis of Enzymatic Activity Based on Temperature
  • 04:25Conclusion

Summary

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The enzymatic activity of lactase is essential for the catabolic processing of the disaccharide lactose. Here, the activity of lactase found in dietary supplements is assayed using a colorimetric assay. This provides students with an experimental platform for understanding the activity of lactase and enzyme kinetics.

Tags

Keywords: LactaseEnzyme ActivityTeaching LabONPGPBSSpectrophotometryTemperatureEnzyme Kinetics

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