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Capitoli
- 00:05Titolo
- 00:38Harvesting S. cerevisiae Cells
- 01:44Lyse Cells and Lysate Clarification
- 04:43Resin Equilibration and Binding and On Column TEV Cleavage
- 06:47Packing and Eluting from Calmodulin Resin
- 08:20Results: TAP Purification of U1 snRNP Complex in S. cerevisiae
- 09:55Conclusion
The Tandem Affinity Purification (TAP) method has been used extensively to isolate native complexes from cellular extract, primarily eukaryotic, for proteomics. Here, we present a TAP method protocol optimized for purification of native complexes for structural studies.
