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Isolierung von Cognate RNA-Protein-Komplexe von Zellen unter Verwendung von Oligonukleotid-gerichtete Elution
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Capitoli
- 00:05Titolo
- 01:14Preparation of Cells and the Affinity Matrix
- 02:31Harvesting RNPs
- 04:14Immnoprecipitation of RNP Complexes
- 05:51Collection of Immunoprecipitated RNA
- 07:32Results: Oligonucleotide-directed Elution of RNA-protein Complexes
- 09:01Conclusion
This manuscript describes an approach to isolate select cognate RNPs formed in eukaryotic cells via a specific oligonucleotide-directed enrichment. We demonstrate the applicability of this approach by isolating a cognate RNP bound to the retroviral 5' untranslated region that is composed of DHX9/RNA helicase A.
