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1Innovation Laboratory, Center for Molecular Oncology,Memorial Sloan Kettering Cancer Center, 2Flow-Sorting Core Facility,Weill Cornell Medical College, 3Department of Laboratory Medicine,University of Washington, 4Department of Physiology and Biophysics, Institute for Computational Biomedicine,Weill Cornell Medical College, 5Department of Pathology and Laboratory Medicine,Weill Cornell Medical College, 6Department of Laboratory Medicine,Memorial Sloan Kettering Cancer Center
Capitoli
- 00:05Titolo
- 01:19Cell Suspension Preparation for Sorting
- 03:00Compensation Control Setup
- 04:53Hodgkin Reed-Sternberg-, B-, and T-cell Isolation
- 06:07Results: Representative DNA Library and Copy Number Gains and Losses Analyses
- 08:02Conclusion
En este trabajo describimos una clasificación combinada de células de citometría de flujo y un protocolo de construcción de biblioteca de próxima generación, de bajo ingreso, diseñado para producir datos de exome de alta calidad de las células Hodgkin Reed-Sternberg (HRS) del linfoma de Hodgkin clásico (CHL).
