Rivista
/
Biologia
/
CUBIC Protocol Visualizes Protein Expression at Single Cell Resolution in Whole Mount Skin Preparations
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
CUBIC Protocol Visualizes Protein Expression at Single Cell Resolution in Whole Mount Skin Preparations

CUBIC Protocol Visualizes Protein Expression at Single Cell Resolution in Whole Mount Skin Preparations

DOI:
10.3791/54401-v

•

08:09 min

•

August 04, 2016

•

, , , , ,
1The School of Medical Sciences,University of New South Wales Australia, 2Neuroscience Research Australia, 3Biomedical Imaging Facility,University of New South Wales Australia

Capitoli

  • 00:05Titolo
  • 01:19Transparent Mouse Skin Tissue Preparation
  • 02:32Mouse Skin Biopsy Clearance
  • 04:27Mouse Skin Biopsy Imaging
  • 05:08Results: Single Cell Resolution Protein Expression in Whole Mount Skin Preparations
  • 06:55Conclusion

Summary

Traduzione automatica

Traduzione automatica

This report describes a CUBIC protocol to clarify full thickness mouse skin biopsies, and visualize protein expression patterns, proliferating cells, and sebocytes at the single cell resolution in 3D. This method enables accurate assessment of skin anatomy and pathology, and of abnormal epidermal phenotypes in genetically modified mouse lines.

Tags

CUBIC ProtocolProtein ExpressionSingle Cell ResolutionWhole Mount Skin PreparationsEpidermal DevelopmentSkin RegenerationSkin DiseaseHair FolliclesSkin BiopsySkin ClearingCUBIC Clearing Solution

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/54401?article_type=v

Method for Whole Mount Antibody Staining in Chick
check_url/it/54401?article_type=v

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
check_url/it/54401?article_type=v

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
check_url/it/54401?article_type=v

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo
check_url/it/54401?article_type=v

Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos
check_url/it/54401?article_type=v

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations
check_url/it/54401?article_type=v

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function
check_url/it/54401?article_type=v

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia
check_url/it/54401?article_type=v

An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis
check_url/it/54401?article_type=v

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy

Read Article