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Udvikling af et antigen-drevet Colitis Model at studere Præsentation af antigener ved antigen-præsenterende celler for T-celler
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells

Udvikling af et antigen-drevet Colitis Model at studere Præsentation af antigener ved antigen-præsenterende celler for T-celler

DOI:
10.3791/54421-v

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06:57 min

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September 18, 2016

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, , ,
1APC Microbiome Institute,University College Cork, 2Division of Gastroenterology and Hepatology,University Hospital Basel, 3Institute of Microbiology and Biotechnology,University of Ulm

Capitoli

  • 00:05Titolo
  • 01:28Colonic Lamina propria Cell Isolation
  • 03:58Results: Host CX3CR1+ Macrophages Communicate with Donor OT-II Cells During OVA-driven Transfer Colitis
  • 06:03Conclusion

Summary

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Traduzione automatica

In this antigen-driven colitis model, OT-II CD4+ T cells expressing a red fluorescent protein were adoptively transferred into RAG-/- mice that express a green fluorescent protein in mononuclear phagocytes (MPs). The hosts were challenged with Escherichia coli (E.coli) expressing the ovalbumin protein (OVA) fused to a cyan fluorescent protein (CFP).

Tags

Keywords: Antigen-driven ColitisAntigen Presenting CellsT CellsInflammatory Bowel DiseaseLamina PropriaT Cell Transfer Colitis ModelMononuclear PhagocytesCD4 T CellsEpithelial Cell Removal

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