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Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection
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JoVE Journal Genetica
Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection

Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection

DOI:
10.3791/54426-v

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10:50 min

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September 27, 2016

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, , ,
1Electrical Engineering,National Tsing Hua University

Capitoli

  • 00:05Titolo
  • 00:46Assembly of the Morbidostat
  • 04:13Pretesting and Running the Morbidostat
  • 06:23On the Chip Growth Experiment
  • 07:38Results: Evolution of Antibiotic Drug Resistance using a Morbidostat
  • 09:31Conclusion

Summary

Traduzione automatica

Traduzione automatica

We describe a low cost, configurable morbidostat that enables the characterization of antibiotic drug resistance by dynamically adjusting the drug concentration. The device can be integrated with a multiplexed microfluidic platform. The approach can be scaled up for laboratory antibiotic drug resistance studies.

Tags

MorbidostatAntibiotic ResistanceAdaptive EvolutionBacteriaLow-costAutomatedElectronic ComponentsPDMSLEDPhoto DetectorMagnetic Stirrer

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