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A Mimic of the Tumor Microenvironment: A Simple Method for Generating Enriched Cell Populations and Investigating Intercellular Communication
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JoVE Journal Ricerca sul cancro
A Mimic of the Tumor Microenvironment: A Simple Method for Generating Enriched Cell Populations and Investigating Intercellular Communication

A Mimic of the Tumor Microenvironment: A Simple Method for Generating Enriched Cell Populations and Investigating Intercellular Communication

DOI:
10.3791/54429-v

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09:52 min

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September 20, 2016

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, ,
1Department of Radiology, New Jersey Medical School,Rutgers University

Capitoli

  • 00:05Titolo
  • 00:52Insert Preparation
  • 03:40Cell Collection and In Situ Immunofluorescence Characterization
  • 06:22Results: Representative Tumor Microenvironment Generation
  • 07:47Conclusion

Summary

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We adapted a permeable microporous membrane insert to mimic the tumor microenvironment (TME). The model consists of a mixed cell culture, allows simplified generation of highly enriched individual cell populations without using fluorescent tagging or cell sorting, and permits studying intercellular communication within the TME under normal or stress conditions.

Tags

Tumor MicroenvironmentIn Vitro Coculture ModelIntercellular CommunicationCancer-associated FibroblastsTherapeutic AgentsCell PopulationsCell CultureCell DissociationCell SeedingCell Incubation

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