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Capitoli
- 00:05Titolo
- 00:56Blocking Replication with FROS
- 02:32Fluorescence Microscopy
- 04:00DNA Extraction/Agarose Plugs
- 06:17Neutral-neutral 2-Dimensional Gel Electrophoresis
- 09:22Results: FROS Creates a Site Specific and Reversible Replecation Blockage
- 10:53Conclusion
We describe here a system utilizing a site-specific, reversible in vivo protein block to stall and collapse replication forks in Escherichia coli. The establishment of the replication block is evaluated by fluorescence microscopy and neutral-neutral 2-dimensional agarose gel electrophoresis is used to visualize replication intermediates.
