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Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing
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JoVE Journal Medicina
Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing

Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing

DOI:
10.3791/54460-v

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07:38 min

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September 13, 2016

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, , , , ,
1Department of Medicine B,University Hospital Münster, 2Institute of Palliative Care,University Hospital Münster, 3Biomedical Technology Center,University of Münster, 4Department of Gastroenterology,Klinikum Bielefeld

Capitoli

  • 00:05Titolo
  • 00:58Wound Healing Assay Preparation and Digital Holographic (DHM) Analysis
  • 03:12Acute Dextran Sodium Sulfate (DSS) Colitis Induction and DHM Analysis
  • 04:24Results: Representative DHM Analyses
  • 05:52Conclusion

Summary

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Accurate assessment of anti-inflammatory effects is of utmost importance for the evaluation of potential new drugs for the treatment of inflammatory bowel disease. Digital holographic microscopy provides assessment of inflammation in murine and human colonic tissue samples as well as automated multimodal evaluation of epithelial wound healing in vitro.

Tags

Digital Holographic MicroscopyQuantitative Phase ImagingIntestinal InflammationEpithelial Wound HealingCaco-2 CellsStain-free ImagingCell CultureWound AreaHEPES BufferLaser IlluminationHologram RecordingQuantitative Phase Image Reconstruction

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