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إعداد وتسليم Microcrystals البروتين في المرحلة مكعب شحمي عن المسلسل الفيمتو ثانية البلورات
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Capitoli
- 00:05Titolo
- 00:54Setting up Crystallization in Syringes and Crystal Detection
- 04:06Sample Consolidation and Titration with 7.9 Monoacylglycerol (MAG)
- 06:32Characterization of Microcrystals
- 07:44Results: Representative Results of Sample Preparation Procedure and Final Protein Structures
- 08:27Conclusion
We describe procedures for the preparation and delivery of membrane protein microcrystals in lipidic cubic phase for serial crystallography at X-ray free-electron lasers and synchrotron sources. These protocols can also be applied for incorporation and delivery of soluble protein microcrystals, leading to substantially reduced sample consumption compared to liquid injection.
