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Single-molekyl Super-Resolution Imaging of phosphatidylinositol 4,5-bisfosfat i plasmamembranen med Novel fluorescerende prober
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Capitoli
- 00:05Titolo
- 01:03Membrane Sheet Preparation and Fixation
- 02:53Photoactivation Localization Microscopy (PALM) Image Acquisition
- 05:40Results: Images of PI(4,5)P2 and iRFP-PAmCherry1-PHPLCδ1 Labeled INS-1 Cells
- 06:56Conclusion
PI (4,5) P 2 regulerer ulike cellulære funksjoner, men nanoskala organisasjon i cellen plasmamembranen er dårlig forstått. Ved merking PI (4,5) P-2 med en to-farge fluorescerende probe sammensmeltet til Pleckstrin Homologi domene, beskriver vi en ny tilnærming for å studere PI (4,5) P a romlig fordeling i plasmamembranen til nanometerskala .
Tags
Keywords: Single-molecule Super-resolution ImagingPhosphatidylinositol 45-bisphosphatePlasma MembraneFluorescent ProbesNanoscale DistributionPhosphoinositide SignalingHigh Spatial ResolutionDetergent-freePlasma Membrane Sheet PreparationPhosphoinositide LabelingSingle-molecule Fluorescence ImagingSingle-molecule LocalizationTIRF MicroscopySMLM
