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"Phagosome 폐쇄 분석은"사용하여 입체적으로 Phagosome 형성을 시각화하는 총 내부 반사 형광 현미경 (TIRFM)
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Capitoli
- 00:05Titolo
- 00:51Red Blood Cell (RBC) Opsonization and Poly-lysine Coverslip Coating
- 02:31Non-covalent SRBC Fixation
- 04:25TIRF Microscopy Phagocytosis Visualization
- 05:23Image Acquisition and Processing
- 07:45Results: F-actin Accumulation at the Site of Phagosome Closure
- 08:51Conclusion
We describe an experimental setup to visualize with unprecedented high resolution phagosome formation and closure in three dimensions in living macrophages, using total internal reflection fluorescence microscopy. It allows monitoring of the base of the phagocytic cup, the extending pseudopods, as well as the precise site of phagosome scission.
