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Avaliação rápida e específica da atividade da peroxidase de halogenação em amostras de sangue enriquecido por leucócitos
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples
DOI:

05:17 min

July 28, 2016

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Capitoli

  • 00:05Titolo
  • 00:36Hypotonic Erythrocyte Lysis
  • 02:20Halogenating Peroxidase Activity Staining
  • 03:31Results: Leukocyte Enrichment and APF-derived Fluorescence in a Rat Blood Micro-sample
  • 04:29Conclusion

Summary

Traduzione automatica

This protocol describes the quick enrichment of leukocytes from small blood samples for a subsequent specific determination of the halogenating peroxidase activity within the cells. The method can be applied to human and non-human material and may contribute to the evaluation of new inflammatory markers.

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