È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 01:14Expression and Purification of Trehalose Synthase Thermoproteus tenax (TreT)
- 04:04Synthesis and Purification of a Trehalose Analogue — Exemplified with the Synthesis of 6-Azido-trehalose (6-TreAz)
- 06:59Results: One-step Synthesis of Trehalose Analogues Using TreT
- 08:14Conclusion
Trehalose analogues are emerging as important molecules for bio(techno)logical and biomedical applications. We describe an optimized protocol for enzymatically synthesizing and purifying trehalose analogues that is simple, efficient, fast, and environmentally friendly. Its application to the rapid production and administration of a probe for the detection of mycobacteria is demonstrated.
