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Methoden zur Bestimmung von Preisen von Glucose und die Oxidation von Fettsäuren in der isolierten Arbeitsrattenherz
JoVE Journal
Biologia
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JoVE Journal Biologia
Methods for the Determination of Rates of Glucose and Fatty Acid Oxidation in the Isolated Working Rat Heart
DOI:

12:24 min

September 28, 2016

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Capitoli

  • 00:05Titolo
  • 01:07Rat Heart Isolation and Cannulation
  • 04:51Measurement of Cardiac Function and Sample Collection
  • 07:17Determination of Myocardial Glucose Oxidation Rate
  • 08:48Determination of Myocardial Oleate Oxidation Rates
  • 10:14Results: Measurements from Two Stressed Hearts
  • 11:40Conclusion

Summary

Traduzione automatica

The following protocol describes the preparation and utilization of buffers for the quantitative measurement of rates of glucose and fatty acid oxidation in the isolated working rat heart. The methods used for sample analysis and data interpretation are also discussed.

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