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Imaging G-protein-koblet receptor-medieret Kemotaksi og dens signaleringshændelser i Neutrofil-lignende HL60-celler
JoVE Journal
Biologia
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JoVE Journal Biologia
Imaging G Protein-coupled Receptor-mediated Chemotaxis and its Signaling Events in Neutrophil-like HL60 Cells

Imaging G-protein-koblet receptor-medieret Kemotaksi og dens signaleringshændelser i Neutrofil-lignende HL60-celler

DOI:
10.3791/54511-v

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08:24 min

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September 14, 2016

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, ,
1Chemotaxis Signal Section, Laboratory of Immunogenetics,National Institute of Allergy and Infectious Diseases, National Institutes of Health

Capitoli

  • 00:05Titolo
  • 01:08Cell Mobility Analysis Device Assembly
  • 03:11Cell Mobility Analysis
  • 06:00Results: Representative Cell Mobility Device Chemotaxis Analysis
  • 07:15Conclusion

Summary

Traduzione automatica

Traduzione automatica

Visuelle kemotaksiassays er afgørende for en bedre forståelse af, hvordan eukaryote celler styrer kemoattraktant-medieret retningsbestemt cellevandring. Her beskriver vi detaljerede metoder til: 1) realtid, høj opløsning overvågning af flere kemotaksiassays, og 2) samtidig visualisering af kemoattraktant gradient og spatiotemporale dynamik signalering begivenheder i neutrofil-lignende HL60 celler.

Tags

Keywords: G Protein-coupled ReceptorChemotaxisSignaling EventsNeutrophil-like HL60 CellsMicrofluidicsCell Mobility Analysis DeviceRPMI 1640 MediumBSACell BiologistsSimultaneous MonitoringReal-time Visualization

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