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通过注射心脏毒素急性骨骼肌再生的诱导

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Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection

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通过注射心脏毒素急性骨骼肌再生的诱导

DOI:

10.3791/54515-v

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07:39 min

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January 01, 2017

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Ombretta Guardiola, Gennaro Andolfi, Mario Tirone³, Francescopaolo Iavarone, Silvia Brunelli, Gabriella Minchiotti

¹Stem Cell Fate Laboratory,Institute of Genetics and Biophysics “A. Buzzati Traverso”, CNR, ²School of Medicine and Surgery,University of Milano-Bicocca, ³Division of Immunology, Transplantation and Infectious Diseases,San Raffaele Scientific Institute

Capitoli

00:05Titolo
00:35Cardiotoxin Injection in the Tibialis Anterior Muscle
01:29Tibialis Anterior Isolation
02:51Fresh Frozen Muscle Technique
04:03Cryostat Sectioning of Frozen Muscles
04:46Hematoxylin & Eosin Stains
05:57Results: Time Course Analysis of Skeletal Muscle Regeneration
07:04Conclusion

Summary

Traduzione automatica

该原稿描述了详细的协议来诱导成年小鼠和肌肉的随后的操作，如解剖，冷冻，切割，常规染色，和肌纤维横截面积分析急性骨骼肌再生。

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