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CD4的制备<sup> +</sup> T细胞通过显微镜分析GD3和GD2神经节苷脂膜表达的
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Preparation of CD4+ T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy
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CD4的制备<sup> +</sup> T细胞通过显微镜分析GD3和GD2神经节苷脂膜表达的

DOI:
10.3791/54569-v

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10:00 min

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November 08, 2016

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1Instituto de Investigación en Ciencias Básicas y Aplicadas, Centro de Investigación en Dinámica Celular, Laboratorio de Glicobiología Humana y Diagnóstico Molecular,Universidad Autónoma del Estado de Morelos

Capitoli

  • 00:05Titolo
  • 01:00Isolation and Activation of Human Naïve CD4+ T Cells
  • 04:22Collection and Fixation of Resting and Activated Naïve CD4+ T Cells
  • 05:34Staining, Mounting and Visualization by Confocal Microscopy
  • 07:47Results: Localization of GD3 and GD2 Gangliosides in Adhered Activated CD4+ T Cells
  • 09:13Conclusion

Summary

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我们描述了在显微镜使用，以确定在静止神经节苷脂和激活的人幼稚CD4 + T细胞的细胞膜表达和定位一个标准抗体染色方案。还使用<40,000个细胞，不需要额外的低输入RNA试剂描述的实时PCR实验。

Tags

Keywords: CD4+ T CellsGD3 GangliosideGD2 GangliosideMembrane ExpressionMicroscopyPeripheral Blood Mononuclear Cells (PBMCs)Negative SelectionAnti-CD3 AntibodyAnti-CD28 AntibodyCell StimulationCell CultureCell Preparation

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