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该<em>果蝇</em>成虫盘瘤模型：基因的表达与肿瘤侵袭性的可视化和定量遗传马赛克

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The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics

Please note that all translations are automatically generated. Click here for the English version.

该<em>果蝇</em>成虫盘瘤模型：基因的表达与肿瘤侵袭性的可视化和定量遗传马赛克

DOI:

10.3791/54585-v

•

10:31 min

•

October 06, 2016

•

Juliane Mundorf, Mirka Uhlirova

¹Institute for Genetics, Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD),University of Cologne

Capitoli

00:05Titolo
01:16Genetic Crosses and Collection of Third Instar Larvae
03:10Dissection of Larvae
04:52Fixing and Staining of Tissues
05:58Tissue Mounting and Quantification of Tumor Invasiveness
07:10Results: Visualization, Tumor Quantification, and Gene Expression
09:04Conclusion

Summary

Traduzione automatica

该协议将演示如何生成的果蝇眼睛/触角成虫盘（EAD）荧光标记，基因定义克隆肿瘤。它介绍了如何从第三龄幼虫，以及如何处理它们的可视化和量化基因表达的变化和肿瘤的侵袭性解剖EAD和大脑。

Tags

Keywords: Drosophila Imaginal Disc Tumor Model Genetic Mosaics Gene Expression Cell Invasiveness Quantification Fluorescent Labeling MARCM FLP-FRT System Dissection Immunostaining Image Analysis

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