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The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics
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Capitoli
- 00:05Titolo
- 01:16Genetic Crosses and Collection of Third Instar Larvae
- 03:10Dissection of Larvae
- 04:52Fixing and Staining of Tissues
- 05:58Tissue Mounting and Quantification of Tumor Invasiveness
- 07:10Results: Visualization, Tumor Quantification, and Gene Expression
- 09:04Conclusion
This protocol demonstrates how to generate fluorescently marked, genetically defined clonal tumors in the Drosophila eye/antennal imaginal discs (EAD). It describes how to dissect the EAD and brain from the third instar larvae and how to process them to visualize and quantify gene expression changes and tumor invasiveness.
