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An In Vitro Model of the Blood-brain Barrier Using Impedance Spectroscopy: A Focus on T Cell-endothelial Cell Interaction
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
An In Vitro Model of the Blood-brain Barrier Using Impedance Spectroscopy: A Focus on T Cell-endothelial Cell Interaction

An In Vitro Model of the Blood-brain Barrier Using Impedance Spectroscopy: A Focus on T Cell-endothelial Cell Interaction

DOI:
10.3791/54592-v

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10:17 min

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December 08, 2016

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, , , , ,
1Department of Neurology,University Hospital Münster, 2Institute of Biochemistry,University of Münster

Capitoli

  • 00:05Titolo
  • 01:57Harvesting MBMECs
  • 03:52Setting Up and Performing TEER Measurement
  • 07:18Results: Changes in TEER as a Primary Measure of MBMEC Dysfunction upon Interaction with T Cells
  • 09:01Conclusion

Summary

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Here, we describe an in vitro murine model of the blood-brain barrier that makes use of impedance cell spectroscopy, with a focus on the consequences on endothelial cell integrity and permeability upon interaction with activated T cells.

Tags

Blood-brain BarrierIn Vitro ModelImpedance SpectroscopyT Cell-endothelial Cell InteractionNeuroimmunologyAutoimmune CNS DisordersEAEPrimary Endothelial Cell CultureT Cell ActivationMultiple SclerosisMBMEC

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