Rivista
/
Immunologia e infezioni
/
通过流式细胞仪大鼠肾脏巨噬细胞的表型特征
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Phenotypic Characterization of Macrophages from Rat Kidney by Flow Cytometry
Please note that all translations are automatically generated. Click here for the English version.

通过流式细胞仪大鼠肾脏巨噬细胞的表型特征

DOI:
10.3791/54599-v

•

07:14 min

•

October 18, 2016

•

, , , , , , , , , ,
1Renal, Vascular and Diabetes Research Lab, IIS-Fundaciòn Jiménez Dìaz,Autonoma University, 2Department of Physiology, Faculty of Medicine,Complutense University, 3Department of Immunology, Centro Nacional de Microbiologìa,Instituto de Salud Carlos III (ISCIII)

Capitoli

  • 00:05Titolo
  • 00:51Kidney Perfusion and Extraction
  • 02:07Kidney Digestion and Single Cell Suspension
  • 03:13Cell Staining and Flow Cytometry
  • 04:29Results: Representative Rat Peritoneal Macrophage Characterization
  • 05:53Conclusion

Summary

Traduzione automatica

Traduzione automatica

该原稿描述的表型，并通过流式细胞从大鼠肾脏定居巨噬细胞的定量分析的详细协议。所得染色的细胞也可以用于其它应用，包括细胞分选，基因表达分析或功能性研究，从而增加在实验模型中获得的信息。

Tags

Keywords: MacrophagesKidneyFlow CytometryCharacterizationPhenotypeM1M2InflammationRenal Disease

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/54599?article_type=v

人类<em在体外承认免疫失衡的早期国家禁止作为筛检工具
check_url/it/54599?article_type=v

从老鼠肠道树突状细胞和巨噬细胞的分离和表征
check_url/it/54599?article_type=v

考试流式细胞仪检测胸腺阳性和阴性选择
check_url/it/54599?article_type=v

通过成像流式细胞仪人单核细胞来源的树突状细胞的特征：两个单核细胞分离方法的比较
check_url/it/54599?article_type=v

荧光激活细胞分选从小鼠骨髓浆树突状细胞的分离纯化
check_url/it/54599?article_type=v

人肺树突状细胞：空间分布与表型鉴定支气管活检采用免疫组化和流式细胞仪
check_url/it/54599?article_type=v

受肥胖相关炎症组织的巨噬细胞的分离、鉴定和纯化
check_url/it/54599?article_type=v

通过流式细胞术分析来自中枢神经系统的小胶质细胞和单核细胞衍生的巨噬细胞
check_url/it/54599?article_type=v

小鼠与人肺泡巨噬细胞的分离和体外培养
check_url/it/54599?article_type=v

全血流式细胞术分析人单核细胞亚群的表征

Read Article