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Nigral डोपामाइन न्यूरॉन्स की Somatodendritic डोमेन से Subcellular पैच दबाना रिकॉर्डिंग
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Capitoli
- 00:05Titolo
- 00:36Preparation of Brain Slices
- 02:11Dendritic Cell-attached Recording
- 05:58Post Hoc Visualization of Biocytin-filled Neurons
- 07:07Results: Cell-attached Recording in a DA Neuron
- 08:13Conclusion
Subcellular patch-clamp recordings offer the possibility to investigate the functional properties of dendrites. However these fine structures are not easily accessible due to their small diameter. The protocol described here aims to facilitate the collection of stable and reliable recordings from the dendrites of dopamine neurons in slices in vitro.
