/

Ricerca sul cancro

/

真空安定化撮像窓と肺における長期高分解能生体顕微鏡

JoVE Journal
Ricerca sul cancro

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Ricerca sul cancro

Long-term High-Resolution Intravital Microscopy in the Lung with a Vacuum Stabilized Imaging Window

Please note that all translations are automatically generated. Click here for the English version.

真空安定化撮像窓と肺における長期高分解能生体顕微鏡

DOI:

10.3791/54603-v

•

07:19 min

•

October 06, 2016

•

Carolina Rodriguez-Tirado, Takanori Kitamura, Yu Kato², Jeffery W. Pollard^2,5, John S. Condeelis⁴, David Entenberg⁴

¹Department of Developmental and Molecular Biology,Albert Einstein College of Medicine, ²Department of Obstetrics/Gynecology and Woman’s Health,Albert Einstein College of Medicine, ³Department of Anatomy & Structural Biology,Albert Einstein College of Medicine, ⁴Gruss-Lipper Biophotonics Center Integrated Imaging Program,Albert Einstein College of Medicine, ⁵Medical Research Council Centre for Reproductive Health, Queen’s Medical Research Institute,University of Edinburgh

Capitoli

00:05Titolo
00:44Lung Lobe Exposure
03:25Intravital Imaging
04:47Results: Representative Intravital Vacuum Stabilized Imaging
06:31Conclusion

Summary

Traduzione automatica

This protocol describes the use of multiphoton microscopy to perform long-term high-resolution, single cell imaging of the intact lung in real time using a vacuum stabilized imaging window.

Tags

Long-term Imaging High-resolution Imaging Intravital Microscopy Lung Vacuum Stabilized Imaging Window Ricerca sul cancro Single-cell Resolution Tracheal Catheter Depilatory Cream Intubation Ventilator Rib Cage Removal Vacuum Window Pulse Oximeter Environmental Chamber

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Ex Vivoひと肺腫瘍内常駐 cd8 陽性 T リンパ球の画像スライス共焦点顕微鏡を用いた

長期的な住セルイメージ投射細胞の運命をパクリタキセルに応答を評価するために

高分解能共焦点顕微鏡による3D腫瘍スフェロイド侵入に対する移動性阻害剤の効果の特徴

腫瘍細胞の横尾静脈注入後の肺転移の病理学的解析

頭蓋内注射と磁気共鳴画像による脳転移のモデル化

透明なシリコーン窓による縦方向の生体内イメージング

肺への癌転移を調査するための永久的な窓

遺伝子改変マウスモデルにおける同所性ATC腫瘍の解析のための高解像度超音波検査

マウス膵臓の生体内イメージングのための安定化ウィンドウ

Two-Photon Intravital Microscopy of Glioblastoma in a Murine Model

Read Article