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I Vivo Study of Human endotel-Pericyte Interaksjon Bruke Matrix Gel Plug-analysen i Mus
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Biologia
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JoVE Journal Biologia
In Vivo Study of Human Endothelial-Pericyte Interaction Using the Matrix Gel Plug Assay in Mouse

I Vivo Study of Human endotel-Pericyte Interaksjon Bruke Matrix Gel Plug-analysen i Mus

DOI:
10.3791/54617-v

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08:16 min

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December 19, 2016

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, , ,
1Division of Pulmonary and Critical Care Medicine, School of Medicine,Stanford University, 2Stanford Cardiovascular Institute, School of Medicine,Stanford University, 3VA Palo Alto Health Care System, Department of Cardiothoracic Surgery, School of Medicine,Stanford University

Capitoli

  • 00:05Titolo
  • 00:56Cell and Matrix Gel Preparation
  • 03:24Matrix Gel Injection
  • 05:05Plug Isolation: 14 Days Post-injection
  • 06:16Result: H&E and Immunofluorescent Staining
  • 07:00Conclusion

Summary

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Vi presenterer en protokoll for å studere humane endotel-pericyte interaksjoner i mus ved anvendelse av en variasjon av matrisen gelen pluggen angiogenese assay.

Tags

In VivoEndothelial-pericyte InteractionMatrix Gel Plug AssayMouseAngiogenesisBlood Vessel FormationSignaling PathwaysVascular DisordersEndothelial CellsPericytesCell CultureMatrigelBeta-FGFTrypsinizationCell CountingCell Injection

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