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Ein CFSE-basierten Assay die Migration von murinen Haut Dendritischen Zellen in Lymphknoten während der Infektion zu studieren mit<em> Mycobacterium bovis</em> Bazillus Calmette-Guérin
JoVE Journal
Immunologia e infezioni
This content is Free Access.
JoVE Journal Immunologia e infezioni
A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-Guérin
DOI:

07:14 min

October 09, 2016

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Capitoli

  • 00:05Titolo
  • 00:56Bacille Calmette-Guérin (BCG) and CFSE Injection
  • 02:08Popliteal Lymph Node (pLN) Harvest, Single Cell Suspension Preparation and Cell Surface Staining
  • 04:05Results: Migratory Skin Dendritic Cell Draining LN Relocation after BCG Footpad Infection
  • 06:04Conclusion

Summary

Traduzione automatica

An assay in mice to track cell migration from skin to draining lymph node is described which enables the characterization of skin Dendritic cells mobilized to the lymph node after footpad infection with Bacille Calmette-Guérin.

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